Resultado da pesquisa (3)
Termo utilizado na pesquisa Okuda L.H.
#1 - Serosurvey of bluetongue, caprine arthritis-encephalitis (CAE) and Maedi-Visna in Barbary sheep (Ammotragus lervia) of a southern Brazilian zoo
Abstract in English:
Bluetongue (BT) is an infectious and non-contagious disease of compulsory notification which may affect domestic and wild ruminants, transmitted by Culicoides spp. midges. Despite the high morbidity and mortality in sheep, role of wild animals in the BT cycle remains unclear. Caprine arthritis-encephalitis (CAE) and Maedi-Visna virus (MVV) have been reportedly found in goats and sheep, but not described in wildlife species. Accordingly, serum samples from 17 captive Barbary sheep (Ammotragus lervia) from Curitiba zoo, southern Brazil, were tested for bluetongue, caprine arthritis-encephalitis (CAE) and Maedi-Visna viruses by agar gel immunodiffusion (AGID) and enzyme linked immunosorbent assay (ELISA). Antibodies for bluetongue were observed in 6/17 (35.3%) Barbary sheep by AGID test and in 7/17 (41.2%) by ELISA. All samples were negative for the presence of antibodies against caprine arthritis-encephalitis (CAE) and Maedi-Visna viruses. These findings indicate that Barbary sheep may be infected by bluetongue virus and act as wildlife reservoir in both captive and free-range environments.
Abstract in Portuguese:
A língua azul é uma doença infecciosa e não contagiosa, de notificação obrigatória, que pode afetar ruminantes domésticos e silvestres, transmitida por mosquitos do gênero Culicoides spp. Apesar da alta morbidade e mortalidade em ovelhas, o papel de animais silvestres no ciclo do vírus da língua azul é desconhecido. A artrite encefalite caprina (CAE) e Maedi-visna vírus (MVV) tem sido encontrados em cabras e ovelhas, porém não há descrição em espécies selvagens. Amostras de soro de 17 aoudads (Ammotragus lervia), mantidos em cativeiro no Zoológico de Curitiba, Sul do Brasil, foram testadas para os vírus da língua azul, da artrite encefalite caprina (CAE) e Maedi-visna, utilizando imunodifusão em gel de ágar e o teste de ELISA (enzyme linked immunosorbent assay). Foram observados anticorpos para o vírus da língua azul em 35,3% (6/17) aoudads utilizando a imunodifusão em gel de ágar e 41,2% (7/17) no ELISA. Todas as amostras foram negativas para a presença de anticorpos contra os vírus da artrite encefalite caprina e Maedi-visna. Esses resultados indicam que os aoudads podem ser infectados pelo vírus da língua azul e atuar como um reservatório silvestre tanto em cativeiro quanto em vida livre.
#2 - Perdas reprodutivas associadas com infecção por Toxoplasma gondii em caprinos no sul do Brasil, p.167-171
Abstract in English:
ABSTRACT.- Pescador C.A., Oliveira E.C., Pedroso P.M.O., Bandarra P.M., Okuda L.H., Corbellini L.G. & Driemeier D. 2007. [Reproductive losses linked to Toxoplasma gondii infection in goats in southern Brazil.] Perdas reprodutivas associadas com infecção por Toxoplasma gondii em caprinos no sul do Brasil. Pesquisa Veterinária Brasileira 27(4):167-171. Departamento de Patologia Clínica Veterinária, Faculdade de Veterinária, Universidade Federal do Rio Grande do Sul, Av. Bento Gonçalves 9090, Porto Alegre, RS 91540-000, Brazil. E-mail: davetpat@ufrgs.br
Toxoplasma gondii was implicated with reproductive losses in a goat herd in Rio Grande do Sul, Brazil. Gross changes were present in 2 out of 6 fetuses/offsprings submitted for diagnosis and included enlarged, pale mesenteric lymph nodes, and edematous, mottled red and tan lungs. Microscopic changes were observed in most fetuses and were especially characterized by lymphoplasmacytic infiltration in the brain and lungs. Other histological changes included lymphoplasmacytic interstitial nephritis, necrotizing lymphadenitis and periportal lympho-plasmacytic cell infiltrates. While bacteriological and direct immunofluorescent antibody tests for Leptospira sp. were negative in samples from all cases, immunohistochemical and PCR procedures for Toxoplasma gondii reacted positively in most of them. Antibody titles against T. gondii varying from 1:512 to 1:2048 were detected in serum samples from the mother goats of these aborted (1), stillborn (3) or dead newborn (2) kids. This paper describes the clinical, pathological, serological, molecular and immunohistochemical findings of a Toxoplasma gondii-infected goat flock.
Abstract in Portuguese:
ABSTRACT.- Pescador C.A., Oliveira E.C., Pedroso P.M.O., Bandarra P.M., Okuda L.H., Corbellini L.G. & Driemeier D. 2007. [Reproductive losses linked to Toxoplasma gondii infection in goats in southern Brazil.] Perdas reprodutivas associadas com infecção por Toxoplasma gondii em caprinos no sul do Brasil. Pesquisa Veterinária Brasileira 27(4):167-171. Departamento de Patologia Clínica Veterinária, Faculdade de Veterinária, Universidade Federal do Rio Grande do Sul, Av. Bento Gonçalves 9090, Porto Alegre, RS 91540-000, Brazil. E-mail: davetpat@ufrgs.br
Toxoplasma gondii was implicated with reproductive losses in a goat herd in Rio Grande do Sul, Brazil. Gross changes were present in 2 out of 6 fetuses/offsprings submitted for diagnosis and included enlarged, pale mesenteric lymph nodes, and edematous, mottled red and tan lungs. Microscopic changes were observed in most fetuses and were especially characterized by lymphoplasmacytic infiltration in the brain and lungs. Other histological changes included lymphoplasmacytic interstitial nephritis, necrotizing lymphadenitis and periportal lympho-plasmacytic cell infiltrates. While bacteriological and direct immunofluorescent antibody tests for Leptospira sp. were negative in samples from all cases, immunohistochemical and PCR procedures for Toxoplasma gondii reacted positively in most of them. Antibody titles against T. gondii varying from 1:512 to 1:2048 were detected in serum samples from the mother goats of these aborted (1), stillborn (3) or dead newborn (2) kids. This paper describes the clinical, pathological, serological, molecular and immunohistochemical findings of a Toxoplasma gondii-infected goat flock.
#3 - Eradication of Herpesvirus Bovine-1 (BHV-1) from a dairy cattle herd with a semi-intensive management., 18(2):65-68
Abstract in English:
ABSTRACT.- Del Fava C., De Stefano E., Pituco E.M., Bilynsky J.M.C.V., Okuda L.H., Pozzi, C.R., Veríssimo C.J. & Demarchi J.J.A.A. 1998. [Eradication of Herpesvirus Bovine-1 (BHV-1) from a dairy cattle herd with a semi-intensive management.] Erradicação do Herpesvírus Bovino- 1 (BHV-1) de um rebanho bovino leiteiro em manejo semi-intensivo. Pesquisa Veterinária Brasileira 18(2):61-68. Instituto de Zootecnia, Cx. Postal 60, Nova Odessa, SP 13460-000, Brazil.
To eradicate the BHV-1 from a dairy cattle herd without vaccination, a preliminar serological survey was performed in 154 animals, that revealed 15.6% seropositives for BHV- 1. The test used was serum neutralization with the microtitration technique. The seropositive nonpregnant cows were immediately eliminated from the herd and the pregnant cows were isolated and eliminated after parturiation. The calves with colostral antibodies were kept in the herd. There were no seropositive animals between the 6 to 12 months old calves and the heifers. The animals were monitored serologically every 3 months for 21 months and afterwords in 2 semestral intervals. The seropositive nonpregnant, pregnant and the nursing cows were the source of BHV-1 infecction. In order to maintain a BHV-1 free herd, measurements of control had been taken, as the utilization of virus-free semen, quarantine when animals were introduced into the herd, and semestral serological surveys. Through these measurements the farm has been BHV-1 free for 18 months.
Abstract in Portuguese:
RESUMO.- Del Fava C., De Stefano E., Pituco E.M., Bilynsky J.M.C.V., Okuda L.H., Pozzi, C.R., Veríssimo C.J. & Demarchi J.J.A.A. 1998. [Eradication of Herpesvirus Bovine-1 (BHV-1) from a dairy cattle herd with a semi-intensive management.] Erradicação do Herpesvírus Bovino- 1 (BHV-1) de um rebanho bovino leiteiro em manejo semi-intensivo. Pesquisa Veterinária Brasileira 18(2):61-68. Instituto de Zootecnia, Cx. Postal 60, Nova Odessa, SP 13460-000, Brazil.
Com o objetivo de erradicar o BHV-1 de um rebanho bovino leiteiro de alto valor genético sem a utilização de vacina, foi realizado um exame sorológico prévio em 154 animais, onde constatou-se 15,6% de reagentes ao BHV-1. A técnica utilizada foi a soroneutralização em microplacas. Dentre os animais soropositivos, as vacas vazias foram descartadas imediatamente e as prenhes isoladas e descartadas após o parto. Os bezerros apresentaram anticorpos colostrais até os seis meses de idade, motivo pelo qual não foram descartados; nos bezerros de 6 a 12 meses de idade e nas novilhas não foram diagnosticados animais soropositivos. Os animais foram examinados trimestralmente, por 21 meses, seguido de mais duas coletas semestrais. As vacas secas, prenhes e em lactação, soropositivas, revelaram ser a fonte de infecção do BHV-1. A manutenção de rebanho livre é possível, desde que sejam adotadas medidas como a utilização de sêmen livre de BHV-1, realização de quarentena no ingresso de animais e exames sorológicos anuais visando impedir a reintrodução do vírus. Com o conjunto destas medidas adotadas, a fazenda encontra-se há 18 meses livre do BHV-1.
